Mechanical stress rapidly induces E-resveratrol and E-piceatannol biosynthesis in grape canes stored as a freshly-pruned byproduct

Kevin Billet (1) , Benjamin Houillé (1) , Sébastien Besseau (1) , Céline Melin (1) , Audrey Oudin (1) , Nicolas Papon (2) , Vincent Courdavault (1) , Marc Clastre (1) , Nathalie Giglioli-Guivarc'H (1) , Arnaud Lanoue (1)

1 BBV - Biomolécules et biotechnologies végétales
2 GEIHP - Groupe d'Étude des Interactions Hôte-Pathogène

Grape canes represent a promising source of bioactive phytochemicals. However the stabilization of the raw material after pruning remains challenging. We recently reported the induction of stilbenoid metabolism after winter pruning including a strong accumulation of E-resveratrol and E-piceatannol during the first six weeks of storage. In the present study, the effect of mechanical wounding on freshly-pruned canes was tested to increase the induction of stilbenoid metabolism. Cutting the grape canes in short segments immediately after pruning triggered a transient expression of phenylalanine ammonia-lyase (PAL) and stilbene synthase (STS) genes, followed by a rapid accumulation of E-resveratrol and E-piceatannol. The degree of stilbenoid induction was related to the intensity of mechanical wounding. Data suggest that a global defense response is triggered involving jasmonate signaling, PR proteins and stilbenoid metabolism. Mechanica

Domaines: Génie des procédés; Biotechnologies; Amélioration des plantes

Kevin Billet, Benjamin Houillé, Sébastien Besseau, Céline Melin, Audrey Oudin, et al.. Mechanical stress rapidly induces E-resveratrol and E-piceatannol biosynthesis in grape canes stored as a freshly-pruned byproduct. Food Chemistry, 2018, 240, pp.1022-1027. ⟨10.1016/j.foodchem.2017.07.105⟩. ⟨hal-02407075⟩