Influence of the carbohydrate-binding module on the activity of a fungal aa9 lytic polysaccharide monooxygenase on cellulosic substrates

Amani Chalak (1, 2) , Ana Villares Garicochea (2) , Céline Moreau (2) , Mireille Haon (1) , Sacha Grisel (1) , Angelina d'Orlando (2) , Isabelle Herpoel-Gimbert (1, 3) , Aurore Labourel (1) , Bernard Cathala (2) , Jean-Guy Berrin (1)

1 BBF - Biodiversité et Biotechnologie Fongiques
2 BIA - Unité de recherche sur les Biopolymères, Interactions Assemblages
3 AMU - Aix Marseille Université

Background: Cellulose-active lytic polysaccharide monooxygenases (LPMOs) secreted by filamentous fungi play a key role in the degradation of recalcitrant lignocellulosic biomass. They can occur as multidomain proteins fused to a carbohydrate-binding module (CBM). From a biotech perspective, LPMOs are promising innovative tools for producing nanocelluloses and biofuels, but their direct action on cellulosic substrates is not fully understood. Results: In this study, we probed the role of the CBM from family 1 (CBM1) appended to the LPMO9H from Podospora anserina (PaLPMO9H) using model cellulosic substrates. Deletion of the CBM1 weakened the binding to cellulose nanofibrils, amorphous and crystalline cellulose. Although the release of soluble sugars from cellulose was drastically reduced under standard conditions, the truncated LPMO retained some activity on soluble oligosaccharides. The cellulolytic action of the truncated LPMO was demonstrated using synergy experiments with a cellobiohydrolase (CBH). The truncated LPMO was still able to improve the efficiency of the CBH on cellulose nanofibrils in the same range as the full-length LPMO. Increasing the substrate concentration enhanced the performance of PaLPMO9H without CBM in terms of product release. Interestingly, removing the CBM also altered the regioselectivity of PaLPMO9H, significantly increasing cleavage at the C1 position. Analysis of the insoluble fraction of cellulosic substrates evaluated by optical and atomic force microscopy confirmed that the CBM1 module was not strictly required to promote disruption of the cellulose network. Conclusions: Absence of the CBM1 does not preclude the activity of the LPMO on cellulose but its presence has an important role in driving the enzyme to the substrate and releasing more soluble sugars (both oxidized and nonoxidized), thus facilitating the detection of LPMO activity at low substrate concentration. These results provide insights into the mechanism of action of fungal LPMOs on cellulose to produce nanocelluloses and biofuels.

Mots clés: filamentous fungi; cellulose; lpmo; cmb; microscopy; cbh; enzymatic hydrolysis; potentiality

Mots clés: trichoderma reesei; podospora anserina; hydrolyse enzymatique; dégradation; microscopie à force atomique; cellobiohydrolase; endoglucanase; cellulose cristalline; potentialité

Domaines: Sciences du Vivant [q-bio]

Amani Chalak, Ana Villares Garicochea, Céline Moreau, Mireille Haon, Sacha Grisel, et al.. Influence of the carbohydrate-binding module on the activity of a fungal aa9 lytic polysaccharide monooxygenase on cellulosic substrates. Biotechnology for Biofuels, 2019, pp.1-10. ⟨10.1186/s13068-019-1548-y⟩. ⟨hal-02405808⟩