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Article Dans Une Revue Methods in Molecular Biology Année : 2017

Probing Activation and Deactivation of the BRASSINOSTEROID INSENSITIVE1 Receptor Kinase by Immunoprecipitation

Résumé

Brassinosteroids (BRs) are sterol-derived hormones that control plant growth and development. The BR receptor complex is encoded by the BRASSINOSTEROID INSENSITIVE1 (BRI1) and members of the SOMATIC EMBRYOGENESIS RECEPTOR KINASE family. For activation and deactivation, the BR receptor complex uses different posttranslational modifications and recruits various partner proteins. Here, we describe optimized immunoprecipitation protocols and variants for biochemical analyses of posttranslational modifications of BRI1 and of protein-protein interactions.

Dates et versions

hal-02390049 , version 1 (02-12-2019)

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Citer

Sara Martins, Grégory Vert, Yvon Jaillais. Probing Activation and Deactivation of the BRASSINOSTEROID INSENSITIVE1 Receptor Kinase by Immunoprecipitation. Methods in Molecular Biology, 2017, 1564, pp.169--180. ⟨10.1007/978-1-4939-6813-8_14⟩. ⟨hal-02390049⟩
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