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Jointly super-resolved and optically sectioned Bayesian reconstruction method for structured illumination microscopy

Abstract : Structured Illumination Microscopy (SIM) is an imaging technique for achieving both super-resolution (SR) and optical sectioning (OS) in wide-field microscopy. It consists in illuminating the sample with periodic patterns at different orientations and positions. The resulting images are then processed to reconstruct the observed object with SR and/or OS. In this work, we present BOSSA-SIM, a general-purpose SIM reconstruction method, applicable to moving objects such as encountered in in vivo retinal imaging, that enables SR and OS jointly in a fully unsupervised Bayesian framework. By modeling a 2-layer object composed of an in-focus layer and a defocused layer, we show that BOSSA-SIM is able to jointly reconstruct them so as to get a super-resolved and optically sectioned in-focus layer. The achieved performance, assessed quantitatively by simulations for several noise levels, compares favorably with a state-of-the-art method. Finally, we validate our method on open-access experimental microscopy data.
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https://hal.archives-ouvertes.fr/hal-02388773
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Submitted on : Monday, December 2, 2019 - 10:17:09 AM
Last modification on : Tuesday, November 16, 2021 - 5:12:46 AM
Long-term archiving on: : Tuesday, March 3, 2020 - 5:20:16 PM

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Yann Lai-Tim, Laurent Mugnier, François Orieux, Roberto Baena-Gallé, Michel Paques, et al.. Jointly super-resolved and optically sectioned Bayesian reconstruction method for structured illumination microscopy. Optics Express, Optical Society of America - OSA Publishing, 2019, 27 (23), pp.33251-33267. ⟨10.1364/OE.27.033251⟩. ⟨hal-02388773⟩

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