Skip to Main content Skip to Navigation

Sensitive and Specific Detection of Ewing Sarcoma Minimal Residual Disease in Ovarian and Testicular Tissues in an In Vitro Model

Abstract : Ewing sarcoma (EWS) is a common pediatric solid tumor with high metastatic potential. Due to toxic effects of treatments on reproductive functions, the cryopreservation of ovarian tissue (OT) or testicular tissue (TT) is recommended to preserve fertility. However, the risk of reintroducing residual metastatic tumor cells should be evaluated before fertility restoration. Our goal was to validate a sensitive and specific approach for EWS minimal residual disease (MRD) detection in frozen germinal tissues. Thawed OT (n = 12) and TT (n = 14) were contaminated with tumor RD-ES cells (10, 100, and 1000 cells) and EWS-FLI1 tumor-specific transcript was quantified with RT-qPCR. All contaminated samples were found to be positive, with a strong correlation between RD-ES cell numbers and EWS-FLI1 levels in OT (r = 0.93) and TT (r = 0.96) (p < 0.001). No transcript was detected in uncontaminated control samples. The invasive potential of Ewing cells was evaluated using co-culture techniques. After co-culturing, tumor cells were detected in OT/TT with histology, FISH, Cancers 2019, 11, 1807 2 of 13 and RT-qPCR. In addition, four OT and four TT samples from children with metastatic EWS were tested, and no MRD was found using RT-qPCR and histology. We demonstrated the high sensitivity and specificity of RT-qPCR to detect EWS MRD in OT/TT samples. Clinical trial: NCT 02400970.
Complete list of metadatas

Cited literature [30 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-02387351
Contributor : Sandra Carlet-Dollet <>
Submitted on : Friday, November 29, 2019 - 4:53:02 PM
Last modification on : Tuesday, March 17, 2020 - 3:37:26 AM

File

Sensitive and Specific Detecti...
Publisher files allowed on an open archive

Identifiers

Citation

Laure Chaput, Victoria Grèze, Pascale Halle, Nina Radosevic-Robin, Bruno Pereira, et al.. Sensitive and Specific Detection of Ewing Sarcoma Minimal Residual Disease in Ovarian and Testicular Tissues in an In Vitro Model. Cancers, MDPI, 2019, 11, pp.1807. ⟨10.3390/cancers11111807⟩. ⟨hal-02387351⟩

Share

Metrics

Record views

58

Files downloads

115