Skip to Main content Skip to Navigation
Journal articles

Water-in-PDMS emulsion templating of highly interconnected porous architectures for 3D cell culture

Abstract : The development of advanced techniques of fabrication of three-dimensional (3D) microenvironments for the study of cell growth and proliferation has become one of the major motivations of material scientists and bioengineers in the past decade. Here, we present a novel residueless 3D structuration technique of poly(dimethylsiloxane) (PDMS) by water-in-PDMS emulsion casting and subsequent curing process in temperature-/pressure-controlled environment. Scanning electron microscopy and X-ray microcomputed tomography allowed us to investigate the impact of those parameters on the microarchitecture of the porous structure. We demonstrated that the optimized emulsion casting process gives rise to large-scale and highly interconnected network with pore size ranging from 500μm to 1.5 mm that turned out to be nicely adapted to 3D cell culture. Experimental cell culture validations were performed using SaOS-2 (osteosarcoma) cell lines. Epifluorescence and deep penetration imaging techniques as two-photon confocal microscopy unveiled information about cell morphology and confirmed a homogeneous cell proliferation and spatial distribution in the 3D porous structure within an available volume larger than 1 cm3. These results open alternative scenarios for the fabrication and integration of porous scaffolds for the development21of 3D cell culture platforms.
Complete list of metadatas

Cited literature [159 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-02379789
Contributor : Laurent Malaquin <>
Submitted on : Friday, November 29, 2019 - 2:08:05 PM
Last modification on : Tuesday, October 20, 2020 - 11:06:59 AM

File

Riesco_25123.pdf
Files produced by the author(s)

Identifiers

Citation

Roberto Riesco, Louisa Boyer, Sarah Blosse, Pauline Lefebvre, Pauline Assemat, et al.. Water-in-PDMS emulsion templating of highly interconnected porous architectures for 3D cell culture. ACS Applied Materials & Interfaces, Washington, D.C. : American Chemical Society, 2019, 11 (32), pp.28631-28640. ⟨10.1021/acsami.9b07564⟩. ⟨hal-02379789⟩

Share

Metrics

Record views

150

Files downloads

145