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Development and Applications of Superfolder and Split Fluorescent Protein Detection Systems in Biology

Abstract : Molecular engineering of the green fluorescent protein (GFP) into a robust and stable variant named Superfolder GFP (sfGFP) has revolutionized the field of biosensor development and the use of fluorescent markers in diverse area of biology. sfGFP-based self-associating bipartite split-FP systems have been widely exploited to monitor soluble expression in vitro, localization, and trafficking of proteins in cellulo. A more recent class of split-FP variants, named « tripartite » split-FP, that rely on the self-assembly of three GFP fragments, is particularly well suited for the detection of protein-protein interactions. In this review, we describe the different steps and evolutions that have led to the diversification of superfolder and split-FP reporter systems, and we report an update of their applications in various areas of biology, from structural biology to cell biology.
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https://hal.archives-ouvertes.fr/hal-02378354
Contributor : Jean-Denis Pedelacq <>
Submitted on : Tuesday, November 24, 2020 - 3:16:56 PM
Last modification on : Thursday, December 10, 2020 - 10:22:43 AM
Long-term archiving on: : Thursday, February 25, 2021 - 8:22:37 PM

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Jean-Denis Pedelacq, Stéphanie Cabantous. Development and Applications of Superfolder and Split Fluorescent Protein Detection Systems in Biology. International Journal of Molecular Sciences, MDPI, 2019, 20 (14), pp.3479. ⟨10.3390/ijms20143479⟩. ⟨hal-02378354⟩

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