Biophysical characterization and structural determination of the potent cytotoxic Psathyrella asperospora lectin

João P. Ribeiro (1) , Mohamed Ali Abol Hassan , Razina Rouf , Evelin Tiralongo , Tom W. May , Christopher J. Day , Anne Imberty (1) , Joe Tiralongo , Annabelle Varrot (1)

1 CERMAV - Centre de Recherches sur les Macromolécules Végétales

A lectin with strong cytotoxic effect on human colon cancer HT29 and monkey kidney VERO cells was recently identified from the Australian indigenous mushroom Psathyrella asperospora and named PAL. We herein present its biochemical and structural analysis using a multidisciplinary approach. Glycan arrays revealed binding preference towards N-acetylglucosamine (GlcNAc) and, to a lesser extent, towards sialic acid (Neu5Ac). Submicromolar and millimolar affinity was measured by surface plasmon resonance for GlcNAc and NeuAc, respectively. The structure of PAL was resolved by X-ray crystallography, elucidating both the protein's amino acid sequence as well as the molecular basis rationalizing its binding specificity.

Domaines: Chimie

João P. Ribeiro, Mohamed Ali Abol Hassan, Razina Rouf, Evelin Tiralongo, Tom W. May, et al.. Biophysical characterization and structural determination of the potent cytotoxic Psathyrella asperospora lectin. Proteins - Structure, Function and Bioinformatics, 2017, 85 (5), pp.969-975. ⟨10.1002/prot.25265⟩. ⟨hal-02378060⟩