Dynamic Succession of Microbiota during Ensiling of Whole Plant Corn Following Inoculation with Lactobacillus buchneri and Lactobacillus hilgardii Alone or in Combination

Abstract : Lactic acid bacteria (LAB) used as silage additives have been shown to improve several fermentation parameters, including aerobic stability. Inoculation with a combination of Lactobacillus buchneri NCIMB40788 and Lactobacillus hilgardii CNCM-I-4785, contributes to an increase in aerobic stability, compared to each strain inoculated independently. To understand the mode of action of the combination on the LAB community, a fermentation-kinetic study was performed on corn. Four treatments, Control, Lb. buchneri, Lb. hilgardii, and a combination of the two strains, were fermented 1, 2, 4, 8, 16, 32, and 64 days. Corn silage inoculated by both strains had a lactate:acetate ratio of 0.59 after 64 days and a higher concentration of lactate than Lb. buchneri. Analysis of the microbiota by 16S and ITS amplicon metasequencing demonstrated that inoculation led to lower bacterial diversity after 1 day, from 129.4 down to 40.7 observed operational taxonomic units (OTUs). Leuconostocaceae represented the dominant population by day 1, with 48.1%. Lactobacillaceae dominated the succession by day 4, with 21.9%. After 32 days, inoculation by both strains had the lowest bacterial alpha diversity level, with 29.0 observed OTUs, compared to 61.3 for the Control. These results confirm the increased fermentation efficiency when the two Lactobacillus strains are co-inoculated, which also led to a specific yeast OTUs diversity profile, with Hannaella as the main OTU.
Document type :
Journal articles
Complete list of metadatas

Cited literature [72 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-02375686
Contributor : Stéphanie Dutilloy <>
Submitted on : Friday, November 22, 2019 - 10:24:09 AM
Last modification on : Wednesday, November 27, 2019 - 1:41:37 AM

File

DROUIN_Microorganisms_2019.pdf
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Pascal Drouin, Julien Tremblay, Frédérique Chaucheyras-Durand. Dynamic Succession of Microbiota during Ensiling of Whole Plant Corn Following Inoculation with Lactobacillus buchneri and Lactobacillus hilgardii Alone or in Combination. Microorganisms, MDPI, 2019, 7 (12), pp.595. ⟨10.3390/microorganisms7120595⟩. ⟨hal-02375686⟩

Share

Metrics

Record views

13

Files downloads

30