Full genome and characterization of a new species of Chryseobacterium with phytopathogenic potential on Vicia faba (Fabaceae; Papilionoidae)

Abstract : Chryseobacteriumis a genus of the Flavobacteriaceae family whose members areusually chemoorganotrophicand form typical yellow-orange colonies due to flexirubin-type pigment. The genus contains more than 100 species from very diverse ecosystems, including freshwater, soil, marine fish, and human hosts.Other sourcesincludelakes, sediments, water cooling systems, drinking water, lactic acid beverages, beer bottling plants, bioreactor sludge, polluted soil, marine sediment and even permafrost. Chryseobacterium strains are associated with a multitude of animal guts and feces-mosquitos, cockroaches, millipedes,penguin guano, freshwater copepods-, but also bird feathers, milk, meat and chicken.In agricultural systems, they were recovered from soils, roots, flowers, or decaying plant material, but none was described as phytopathogen so far.C.indologenes, mainly found in soil and water, has been reported as uncommon though deadly human pathogen.Here, we report the complete genome sequence and the phenotype characterization of a new strain and new species of Chryseobacterium with a clear though weak phytopathogenic potentialon Vicia faba. Initially detected in an aphid-associated V.fabaseedling growth cabinet, itinduced typicalsoft rot in all aphid-harboringplants.The causal agent was identified as Chryseobacteriumsp.,close to C. indologenes by 16S amplicon sequencing, but not identical to anycharacterizedspecies (databasesin early 2019). Thisprompted us to characterize the F3 strain and determine its genomesequence.The genome of strain F3consists of a circular chromosome of 5,277Kbwithout plasmid. The main phenotypic traits of strain F2 are heavy enzyme secretion, among whichpectinasesand proteases, no direct transmission by aphids at notable frequency but showing increased symptoms on aphid-infected plants, and potential migration through xylem pathway from crown to apex during in planta artificial infection.Very recently, this strain was shown to belong to the samespecies as strain JV274.1, a PGPR strain on maize, which was characterized by French colleagues (Vacheron et al.2017), and soon-to-be published as a novel species(Muller et al. in prep).[1] Rahbé, Y., Guschinskaya, N., Droux, M., Valentovich, L., Briolay, J., & Cotte-Pattat, N. (2019). Full genome and characterization of Chryseobacterium xx, a new phytopathogenic strain with pectinolytic, xylem-association and insect interactions. Systematic and Applied Microbiology 2019, InPrep.
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Yvan Rahbé, Michel Droux, Philippe Oger, Nicole Cotte-Pattat. Full genome and characterization of a new species of Chryseobacterium with phytopathogenic potential on Vicia faba (Fabaceae; Papilionoidae). 14e Rencontres Plantes-Bactéries Aussois 2020, Jan 2020, Aussois, France. ⟨hal-02373109⟩

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