, Zwitterionic acid labile surfactant ZALS I
, Ammonium bicarbonate (NH4HCO3)
, ZUT buffer : 0.1% ZALS I solubilised in 50 mM NH4HCO3, 6M urea, 2M thiourea, 30 mM Tris-HCl
,
, Sequencing grade modified trypsin
, Trypsin digestion: trypsin/protein ratio: 1/30
, Trifluoroacetic acid
, ! CAUTION Formaldehyde solutions and formaldehyde vapors are toxic; prepare solutions in a fume hood
, Formaldehyde solution for molecular biology
, Formaldehyde (CD2O) (20%, 98% D)
, Formaldehyde (13CD2O) (20%, 99% 13C, 98% D)
, Sodium cyanoborohydride (NaBH3CN)
, Sodium cyanoborodeuteride (NaBD3CN)
, 5: mix 1mL of 50mM NaH2PO4 with 3.5 mL of 50 mM Na2 HPO4; Sodium phosphate monobasic (NaH2PO4). Sodium phosphate dibasic
, Light labelling solution: Add 5% (v/v) formaldehyde 4%, 5% (v/v) cyanoborohydride 0, 6M in 50mM sodium phosphate buffer pH 7.5
Protein Kinases and Phosphatases: The Yin and Yang of Protein Phosphorylation and Signaling, Cell, vol.80, pp.225-236, 1995. ,
Cold Acclimation Induces Changes in Arabidopsis Tonoplast Protein Abundance and Activity and Alters Phosphorylation of Tonoplast Monosaccharide Transporters, The Plant Journal, vol.69, pp.529-541, 2012. ,
Phosphoproteome dynamics upon changes in plant water status reveal early events associated with rapid growth adjustment in maize leaves, Mol Cell Proteomics, vol.11, pp.957-72, 2012. ,
Phosphorylation Variation during the Cell Cycle Scales with Structural Propensities of Proteins, PLoS Computational Biology, vol.9, p.1002842, 2013. ,
Regulation of Yeast Central Metabolism by Enzyme Phosphorylation, Molecular Systems Biology, vol.8, p.623, 2012. ,
Post-Translational Modification of Mitochondria as a Novel Mode of Regulation, Experimental Gerontology, vol.56, pp.202-222, 2014. ,
Evaluation and Properties of the Budding Yeast Phosphoproteome, Molecular & Cellular Proteomics, vol.11, 2012. ,
Use of (32) P to study dynamics of the mitochondrial phosphoproteome, J Proteome Res, vol.8, pp.2679-95, 2009. ,
Analysis of steady-state protein phosphorylation in mitochondria using a novel fluorescent phosphosensor dye, J Biol Chem, vol.278, pp.27251-27256, 2003. ,
Mitochondrial phosphoproteome revealed by an improved IMAC method and MS/MS/MS, Mol. Cell. Proteomics, vol.6, pp.669-676, 2007. ,
Tandem metal-oxide affinity chromatography for enhanced depth of phosphoproteome analysis, J Proteomics, vol.75, pp.4602-4611, 2012. ,
Comparison of ERLIC-TiO2, HILIC-TiO2, and SCX-TiO2 for Global Phosphoproteomics Approaches, Journal of Proteome Research, vol.10, pp.3474-3483, 2011. ,
A Strategy for Identification of Protein Tyrosine Phosphorylation, Methods, vol.56, pp.275-283, 2012. ,
Detection and Analysis of Protein Histidine Phosphorylation, Molecular and Cellular Biochemistry, vol.329, pp.93-106, 2009. ,
Stable Isotope Labeling by Amino Acids in Cell Culture, SILAC, as a Simple and Accurate Approach to Expression Proteomics, Molecular & Cellular Proteomics, vol.1, pp.376-386, 2002. ,
Quantitative mitochondrial phosphoproteomics using iTRAQ on an LTQ-Orbitrap with high energy collision dissociation, J Proteome Res, vol.8, pp.4665-75, 2009. ,
Multiplex Peptide Stable Isotope Dimethyl Labeling for Quantitative Proteomics, Nature Protocols, vol.4, pp.484-494, 2009. ,
An MRM-based workflow for quantifying cardiac mitochondrial protein phosphorylation in murine and human tissue, Methods Mol. Biol, vol.1072, pp.621-653, 2014. ,
Quantitative variations of the mitochondrial proteome and phosphoproteome during fermentative and respiratory growth in Saccharomyces cerevisiae, J. Proteomics, vol.106, pp.140-150, 2014. ,
Purification of Saccharomyces cerevisiae Mitochondria Devoid of Microsomal and Cytosolic Contaminations, Anal. Biochemistry, vol.287, pp.339-342, 2000. ,
Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form, Anal. Biochem, vol.199, pp.223-231, 1991. ,
TANDEM: matching proteins with tandem mass spectra, Bioinformatics, vol.20, pp.1466-1467, 2004. ,
MassChroQ: A versatile tool for mass spectrometry quantification, Proteomics, vol.11, pp.3572-3577, 2011. ,
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