, Zwitterionic acid labile surfactant ZALS I

, Ammonium bicarbonate (NH4HCO3)

, ZUT buffer : 0.1% ZALS I solubilised in 50 mM NH4HCO3, 6M urea, 2M thiourea, 30 mM Tris-HCl

K. 2d-quant,

, Sequencing grade modified trypsin

, Trypsin digestion: trypsin/protein ratio: 1/30

, Trifluoroacetic acid

, ! CAUTION Formaldehyde solutions and formaldehyde vapors are toxic; prepare solutions in a fume hood

, Formaldehyde solution for molecular biology

, Formaldehyde (CD2O) (20%, 98% D)

, Formaldehyde (13CD2O) (20%, 99% 13C, 98% D)

, Sodium cyanoborohydride (NaBH3CN)

, Sodium cyanoborodeuteride (NaBD3CN)

, 5: mix 1mL of 50mM NaH2PO4 with 3.5 mL of 50 mM Na2 HPO4; Sodium phosphate monobasic (NaH2PO4). Sodium phosphate dibasic

, Light labelling solution: Add 5% (v/v) formaldehyde 4%, 5% (v/v) cyanoborohydride 0, 6M in 50mM sodium phosphate buffer pH 7.5

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