2–5A synthetase activity does not increase in interferon-resistant friend leukemia cell variants treated with α/β interferon despite the presence of high-affinity interferon receptor sites

E. Affabris , G. Romeo (1) , F. Belardelli (2) , C. Jemma , Nadir Mechti (3) , I. Gresser , G.B. Rossi

1 IACR - International Agency for Cancer Research
2 ISS - Istituto Superiore di Sanità = National Institute of Health
3 IRIM - Institut de Recherche en Infectiologie de Montpellier

The presence of interferon (IFN) receptors on mouse Friend leukemia cells (FLC) has been investigated in binding experiments with highly purified 125I-labeled mouse alpha/beta IFN. Both IFN-resistant clones and wild-type IFN-sensitive FLC showed a specific saturable binding site for mouse IFN with a similar affinity constant. In contrast to IFN-sensitive FLC, IFN-resistant FLC variants were not inducible by IFN for double-stranded RNA-dependent 2-5A synthetase activity.

Domaines: Sciences du Vivant [q-bio]

E. Affabris, G. Romeo, F. Belardelli, C. Jemma, Nadir Mechti, et al.. 2–5A synthetase activity does not increase in interferon-resistant friend leukemia cell variants treated with α/β interferon despite the presence of high-affinity interferon receptor sites. Virology, 1983, 125 (2), pp.508-512. ⟨10.1016/0042-6822(83)90224-6⟩. ⟨hal-02361168⟩