Increased rate of degradation of c-myc mRNA in interferon-treated Daudi cells.

C. Dani (1) , Nadir Mechti (2) , Marc Piechaczyk (3) , B. Lebleu (3) , P. Jeanteur (3) , J. Blanchard

1 IBDC - Institut de signalisation, biologie du développement et cancer - Institute of Developmental Biology and Cancer
2 IRIM - Institut de Recherche en Infectiologie de Montpellier
3 IGMM - Institut de Génétique Moléculaire de Montpellier

The recent observation made in our laboratory that cellular myc (c-myc) mRNA has a very short half-life in a variety of normal and transformed human cells emphasized the potential importance of post-transcriptional regulation of c-myc gene expression. Jonak and Knight [Jonak, G. J. & Knight, E., Jr. (1984) Proc. Natl. Acad. Sci. USA 81, 1747-1750] have reported a selective reduction of c-myc mRNA accumulation in lymphoblastoid Daudi cells treated with human beta interferon. This provided a suitable situation in which to examine a possible action of negative modulators of c-myc expression at the level of mRNA stability. Our results confirm the observation by Jonak and Knight that c-myc mRNA level is depressed in cells treated with beta interferon and extend it to alpha 2 interferon. Furthermore, we now demonstrate that interferon has no effect on c-myc transcription rate in isolated nuclei but rather reduces the half-life of its mRNA. Conversely, we show that it increases the level of HLA-A2 mRNA by stimulating its transcription.

Domaines: Sciences du Vivant [q-bio]

C. Dani, Nadir Mechti, Marc Piechaczyk, B. Lebleu, P. Jeanteur, et al.. Increased rate of degradation of c-myc mRNA in interferon-treated Daudi cells.. Proceedings of the National Academy of Sciences of the United States of America, 1985, 82 (15), pp.4896-4899. ⟨10.1073/pnas.82.15.4896⟩. ⟨hal-02361138⟩