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Article Dans Une Revue Human Molecular Genetics Année : 2020

Sigma-1 receptor is a key genetic modulator in amyotrophic lateral sclerosis

Résumé

Sigma-1 receptor (S1R) is an endoplasmic reticulum (ER) chaperone that regulates mitochondrial respiration but also controls cellular defense against ER and oxidative stress. This makes S1R a potential therapeutic target in amyotrophic lateral sclerosis (ALS). Especially, as a missense mutation E102Q in S1R has been reported in few familial ALS cases. However, the pathogenicity of S1RE102Q and the beneficial impact of S1R in the ALS context remain to be demonstrated in vivo. To address this, we generated transgenic Drosophila that express human wild-type S1R or S1RE102Q. Expression of mutant S1R in fly neurons induces abnormal eye morphology and locomotor defects in a dose-dependent manner. This was accompanied by abnormal mitochondrial fragmentation, reduced ATP levels and a higher fatigability at the neuromuscular junction during high energy demand. Overexpressing IP3 receptor or glucose transporter mitigates the S1RE102Q-induced eye phenotype, further highlighting the role of calcium and energy metabolism in its toxicity. More importantly, we showed that wild-type S1R rescues locomotor activity and ATP levels of flies expressing the key ALS protein, TDP43. Moreover, overexpressing wild-type S1R enhances resistance of flies to oxidative stress. Therefore, our data provide the first genetic evidence that mutant S1R recapitulates ALS pathology in vivo while increasing S1R confers neuroprotection against TDP43 toxicity.
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Dates et versions

hal-02360092 , version 1 (16-11-2020)

Identifiants

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Simon Couly, Bilal Khalil, Véronique Viguier, Julien Roussel, Tangui Tm Maurice, et al.. Sigma-1 receptor is a key genetic modulator in amyotrophic lateral sclerosis. Human Molecular Genetics, 2020, 29 (4), pp.529-540. ⟨10.1093/hmg/ddz267⟩. ⟨hal-02360092⟩
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