Performance of the automated multiplex PCR Unyvero implant and tissue infections system in the management of diabetic foot osteomyelitis

Abstract : AIM: We evaluated the performance of Unyvero implant and tissue infections system (ITI) application (Curetis) to diagnose Diabetic Foot Osteomyelitis (DFOM). PATIENTS & METHODS: The study was conducted in the Diabetic Foot reference center of N\ⁱmes University Hospital (France) from 1 December 2016 to 31 May 2017. We compared the Unyvero ITI PCR to conventional culture and alternative molecular approaches. RESULTS: A total of 79 patients with DFOM were included: 177 microorganisms were isolated by culture, 146 detected by PCR, resulting in a concordance level of 66.7% (65.0-68.4). Discrepant results were obtained for 45 samples, with 59 microorganisms being detected by PCR only (18 samples) or by culture only (27 samples). CONCLUSION: Unyvero ITI PCR represents an interesting additional diagnosis solution to manage DFOM.
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Submitted on : Tuesday, November 12, 2019 - 3:12:27 PM
Last modification on : Friday, November 15, 2019 - 3:04:03 PM

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Catherine Dunyach-Remy, Charlotte Carrere, Hélène Marchandin, Sophie Schuldiner, Anne-Marie Guedj, et al.. Performance of the automated multiplex PCR Unyvero implant and tissue infections system in the management of diabetic foot osteomyelitis. Future Microbiology, Future Medicine, 2018, 13, pp.1669--1681. ⟨10.2217/fmb-2018-0213⟩. ⟨hal-02359778⟩

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