A Spatiotemporal Sequence of Sensitization to Slits and Semaphorins Orchestrates Commissural Axon Navigation

Abstract : Accurate perception of guidance cues is crucial for cell and axon migration. During initial navigation in the spinal cord, commissural axons are kept insensitive to midline repellents. Upon midline crossing in the floor plate, they switch on responsiveness to Slit and Semaphorin repulsive signals and are thus propelled away and prevented from crossing back. Whether and how the different midline repellents control specific aspects of this navigation remain to be elucidated. We set up a paradigm for live-imaging and super-resolution analysis of PlexinA1, Neuropilin-2, and Robo1/2 receptor dynamics during commissural growth cone navigation in chick and mouse embryos. We uncovered a remarkable program of sensitization to midline cues achieved by unique spatiotemporal sequences of receptor allocation at the growth-cone surface that orchestrates receptor-specific growth-cone behavior changes. This reveals post-translational mechanisms whereby coincident guidance signals are temporally resolved to allow the generation of specific guidance responses.
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https://hal.archives-ouvertes.fr/hal-02354488
Contributor : Julien Falk <>
Submitted on : Thursday, November 7, 2019 - 5:27:28 PM
Last modification on : Tuesday, November 19, 2019 - 2:37:00 AM

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Aurora Pignata, Hugo Ducuing, Leila Boubakar, Thibault Gardette, Karine Kindbeiter, et al.. A Spatiotemporal Sequence of Sensitization to Slits and Semaphorins Orchestrates Commissural Axon Navigation. Cell Reports , Elsevier Inc, 2019, 29 (2), pp.347-362.e5. ⟨10.1016/j.celrep.2019.08.098⟩. ⟨hal-02354488⟩

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