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Communication Dans Un Congrès Année : 2019

TGF-BETA PATHWAY IN DEVELOPMENT OF THE SPONGE HALISARCA DUJARDINI.

Résumé

Transforming growth factor beta pathway is one of the major signalling mechanisms that manage development of multicellular organisms together with Wnt, Hedgehog, Delta/Notch, tyrosine-kinase pathways. Axial patterning in embryo development is the brightest example of its role. Functions of TGF-beta pathway established early in evolution of multicellularity as ligands or receptors not found in Protista, whereas in Cnidaria this pathway determines the directive axis of the body. At the same time, TGF-beta expression data from sponges' development tell us about involvement of TGF-beta pathway in axial patterning of Porifera embryo. Number of ligands may be as many as eight in demosponge Amphimedon queenslandica or twenty-two in calcisponge Sycon ciliatum so sponges are not simple in sense of ligand repertoire in comparison to Bilateria. In this study we search through genome, analyzed in silica, cloned and described expression of ligands and receptors of TGF-beta superfamily in demosponge Halisarca dujardini. Eight ligands were identified, and their primary structure and domain organization correspond to eumetazoan TGF-beta. Three of eight identified ligands can be classified as TGF-beta sensu stricto when next five are not fall into any of eumetazoan family TGF-beta. Similar situation described for Wnt pathway ligands in sponges, and it shows independent involvement of some signalling pathways members inside Porifera phyla. Ouantitation of RNAseq data reveals increased expression levels of ligands in osculum of adult sponge. Asymmetric localization of ligand transcripts was described during embryogenesis. Our data suppose that TGF-beta pathway involved in axial patterning in as early branching Metazoa as sponges. The study of long non coding RNAs (lncRNAs) from an evolutionary perspective has been hindered by their lack of strong sequence conservation, apparent lack of secondary structure conservation, and their massive genomic generation and decay rate. One of the most promising approaches has been the search for syntenic lncRNAs over close species. Here, we have developed a pipeline called LincOFinder that thanks to a microsynteny conservation approach, allows us to find conserved clusters of microsynteny between distant organisms surrounding an intergenic lncRNA (lincRNA). Furthermore, we have used this tool to study the conservation and evolution of the lincRNAs repertoire in the chordate lineage, taking advantage of the recently published amphioxus transcriptome and the well annotated human ncRNA dataset. In this way, we are able to identify a putatively conserved set of lincRNAs across chordates, underscoring the case of HOTAIRM1. Here we assess the conservation of this mechanism in vertebrates, and the conservation of the expression domain in chordates, to infer that HOTAIRM1 is highly likely to be conserved across Chordates. Overall, our work pioneers the use of syntenic searches to identify non-coding potential regulatory elements over long evolutionary distances, and helps to further understand lincRNA evolution in the frame of the invertebrate-vertebrate transition.
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Dates et versions

hal-02354371 , version 1 (12-11-2019)

Identifiants

  • HAL Id : hal-02354371 , version 1

Citer

Ilya Borisenko, Andrey Lavrov, Alexander Ereskovsky. TGF-BETA PATHWAY IN DEVELOPMENT OF THE SPONGE HALISARCA DUJARDINI.. European Developmental Biology Congress, Oct 2019, Alicante, Spain. ⟨hal-02354371⟩
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