Characterization of a tRNALys(CUU) gene located in the opposite orientation upstream of a ZmFer2 ferritin gene in the maize nuclear genome

Abstract : The first evidence for a plant tRNA(Lys)(CUU) gene is reported. This gene is found closely linked 400 bp upstream, and on the complementary strand, of a ZmFer2 ferritin gene in the maize nuclear genome. Southern blot analysis indicates that this tRNA(Lys) is a member of a multigene family. This gene does not contain any intron, and exhibits classical intragenic regulatory elements found in eukaryotic tRNA genes (A and B boxes). Moreover, 5' and 3'-flanking sequences display typical features found in nuclear encoded tRNAs. The deduced mature tRNA sequence is almost identical to the sequence of a cytoplasmic tRNA(Lys)(CUU) from wheat germ. The maize tRNA(Lys) gene is expressed in vivo in maize and in transgenic tobacco, as shown by RT-PCR analysis.
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https://hal.archives-ouvertes.fr/hal-02353876
Contributor : Isabelle Fobis-Loisy <>
Submitted on : Thursday, November 7, 2019 - 2:51:52 PM
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Isabelle Fobis-Loisy, Jean-François Briat. Characterization of a tRNALys(CUU) gene located in the opposite orientation upstream of a ZmFer2 ferritin gene in the maize nuclear genome. Gene, Elsevier, 1996, 182 (1-2), pp.195-201. ⟨10.1016/s0378-1119(96)00553-7⟩. ⟨hal-02353876⟩

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