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Transformation from committed progenitor to leukaemia stem cell initiated by human cells. BG and TH interpreted the data and wrote the manuscript ,
, MT's present address is: McGill Cancer Center
, 1 mM PMSF, and a Complete Protease Inhibitor Mixture tablet [Roche]). For immunoprecipitations, cell lysates were incubated with the SCL/TAL1 (C-21) sc-12984 goat polyclonal antibody or with the c-MYC (9E10) sc-40 mouse monoclonal antibody for 2 hours, followed by a 1-hour incubation with protein A-Sepharose CL-4B beads (GE Healthcare), 2× reducing sample buffer (125 mM Tris-HCl, p.4
, The theoretical maximum value for the Z? factor is 1, and ideally the Z? factor is between 0.5 and 1. We performed statistical analyses by Student's t test (*P ? 0.05, **P ? 0.01, ***P ? 0.001). We assumed normality and equal distribution of variance between the different groups analyzed. Survival in mouse experiments was represented with Kaplan-Meier curves, and significance was estimated with log-rank test (GraphPad Prism). Study approval. All AML and T-ALL samples were collected with informed consent according to procedures of the Banque de Cellules Leucémiques du Québec, and the project was approved by the Research Ethics Boards of Maisonneuve-Rosemont Hospital and the University of Montreal, Statistics. Z? value was calculated according to the formula 1 -[3 × (SD of positive control + SD of negative control)/(mean of positive controlmean of negative control)
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