Element content and expression of genes of interest in guard cells are connected to spatiotemporal variations in stomatal conductance

Abstract : Element content and expression of genes of interest on single cell types, such as stomata, provide valuable insights into their specific physiology, improving our understanding of leaf gas exchange regulation. We investigated how far differences in stomatal conductance (g(s)) can be ascribed to changes in guard cells functioning in amphistomateous leaves. g(s) was measured during the day on both leaf sides, on well-watered and drought-stressed trees (two Populus euramericana Moench and two Populus nigra L. genotypes). In parallel, guard cells were dissected for element content and gene expressions analyses. Both were strongly arranged according to genotype, and drought had the lowest impact overall. Normalizing the data by genotype highlighted a structure on the basis of leaf sides and time of day both for element content and gene expression. Guard cells magnesium, phosphorus, and chlorine were the most abundant on the abaxial side in the morning, where g(s) was at the highest. In contrast, genes encoding H+-ATPase and aquaporins were usually more abundant in the afternoon, whereas genes encoding Ca2+-vacuolar antiporters, K+ channels, and ABA-related genes were in general more abundant on the adaxial side. Our work highlights the unique physiology of each leaf side and their analogous rhythmicity through the day.
Document type :
Journal articles
Complete list of metadatas

Cited literature [114 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-02352487
Contributor : Archive Ouverte Prodinra <>
Submitted on : Wednesday, November 6, 2019 - 8:07:40 PM
Last modification on : Saturday, November 9, 2019 - 2:04:18 AM

File

2019_Durand_et_al-2019-Plant,_...
Explicit agreement for this submission

Identifiers

Citation

Maxime Durand, David Cohen, Nathalie Aubry, Cyril Bure, Ivana Tomášková, et al.. Element content and expression of genes of interest in guard cells are connected to spatiotemporal variations in stomatal conductance. Plant, Cell and Environment, Wiley, 2019, ⟨10.1111/pce.13644⟩. ⟨hal-02352487⟩

Share

Metrics

Record views

28

Files downloads

14