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A Subunit of Yeast TFIIIC Participates in the Recruitment of TATA-Binding Protein

Abstract : TFIIIC plays a key role in nucleating the assembly of the initiation factor TFIIIB on class III genes. We have characterized an essential gene, TFC8, encoding the 60-kDa polypeptide, tau60, present in affinity-purified TFIIIC. Hemagglutinin-tagged variants of tau60 were found to be part of TFIIIC-tDNA complexes and to reside at least in part in the downstream DNA-binding domain tauB. Unexpectedly, the thermosensitive phenotype of N-terminally tagged tau60 was suppressed by overexpression of tau95, which belongs to the tauA domain, and by two TFIIIB components, TATA-binding protein (TBP) and B"/TFIIIB90 (but not by TFIIIB70). Mutant TFIIIC was deficient in the activation of certain tRNA genes in vitro, and the transcription defect was selectively alleviated by increasing TBP concentration. Coimmunoprecipitation experiments support a direct interaction between TBP and tau60. It is suggested that tau60 links tauA and tauB domains and participates in TFIIIB assembly via its interaction with TBP.
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Contributor : Eric Deprez <>
Submitted on : Wednesday, November 6, 2019 - 10:00:57 AM
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Eric Deprez, Rosalía Arrebola, Christine Conesa, André Sentenac. A Subunit of Yeast TFIIIC Participates in the Recruitment of TATA-Binding Protein. Molecular and Cellular Biology, American Society for Microbiology, 1999, 19 (12), pp.8042-8051. ⟨10.1128/mcb.19.12.8042⟩. ⟨hal-02350721⟩



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