Skip to Main content Skip to Navigation
Journal articles

A cell cycle-coordinated Polymerase II transcription compartment encompasses gene expression before global genome activation

Abstract : Most metazoan embryos commence development with rapid, transcriptionally silent cell divisions, with genome activation delayed until the mid-blastula transition (MBT). However, a set of genes escapes global repression and gets activated before MBT. Here we describe the formation and the spatio-temporal dynamics of a pair of distinct transcription compartments, which encompasses the earliest gene expression in zebrafish. 4D imaging of pri-miR430 and zinc-finger-gene activities by a novel, native transcription imaging approach reveals tran-scriptional sharing of nuclear compartments, which are regulated by homologous chromosome organisation. These compartments carry the majority of nascent-RNAs and active Polymerase II, are chromatin-depleted and represent the main sites of detectable transcription before MBT. Transcription occurs during the S-phase of increasingly permissive cleavage cycles. It is proposed, that the transcription compartment is part of the regulatory architecture of embryonic nuclei and offers a transcriptionally competent environment to facilitate early escape from repression before global genome activation.
Document type :
Journal articles
Complete list of metadatas

Cited literature [70 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-02349535
Contributor : Laszlo Tora <>
Submitted on : Monday, November 9, 2020 - 11:17:48 AM
Last modification on : Thursday, November 19, 2020 - 9:51:33 AM

File

s41467-019-08487-5.pdf
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Yavor Hadzhiev, Haseeb Qureshi, Lucy Wheatley, Ledean Cooper, Aleksandra Jasiulewicz, et al.. A cell cycle-coordinated Polymerase II transcription compartment encompasses gene expression before global genome activation. Nature Communications, Nature Publishing Group, 2019, 10 (1), ⟨10.1038/s41467-019-08487-5⟩. ⟨hal-02349535⟩

Share

Metrics

Record views

309

Files downloads

2