Cell cycle arrest is sufficient for p53-mediated tumor regression

L Dubrez , J-L Coll (1) , A Hurbin , F de Fraipont , S Lantejoul , M-C Favrot

1 Institut Albert Bonniot - Equipe INSERM 9924 [La Tronche]

p53 gene therapy can induce tumor regression, but the low efficacy of in vivo gene transfer has greatly hampered the mechanistic analysis of this antitumoral activity. We therefore used a p53-null human NSCLC cell line in which we reintroduced the wild-type p53 gene under control of a tetra-cycline-dependent promoter. P53 induction provokes cell cycle arrest in G 0 /G 1 and G 2 /M phase, an up-regulation of p21, a down-regulation of cyclin B1 and appearance of sen-escence features without down-regulation of human telomer-ase reverse transcriptase. No detectable morphological

Mots clés: p53; human lung cancer; senescence; apoptosis; cell cycle arrest; gene therapy; tumor regression

Domaines: Sciences du Vivant [q-bio]

L Dubrez, J-L Coll, A Hurbin, F de Fraipont, S Lantejoul, et al.. Cell cycle arrest is sufficient for p53-mediated tumor regression. Gene Therapy, 2001, 8, pp.1705 - 1712. ⟨hal-02349477⟩