Near-infrared fluorescence imaging-guided surgery improves recurrence-free survival rate in novel orthotopic animal model of head and neck squamous cell carcinoma
Résumé
Background. Appropriate animal models are required to test novel therapeutics for head and neck squamous cell carcinoma (HNSCC) such as near-infrared (NIR) imaging-guided surgery. Methods. We developed an optimized animal model of orthotopic HNSCC (in female athymic NMRI (Naval Medical Research Institute) nude mice) with a prolonged survival time. Resection of the orthotopic tumors was performed 30 days after implantation with or without the aid of a minia-turized clinical grade NIR optical imaging device, after systemic administration of a fluorescent RGD-based probe that targets a v b 3 integrin. Results. NIR optical imaging-guided surgery increased the recurrence-free survival rate by 50% through the detection of fluorescent cancer residues as small as 185 mm; these fragments could remain unidentified if resection was performed exclusively under unaided visual guidance. Conclusion. NIR optical imaging-guided surgery showed an improved HNSCC tumor resection quality in our optimized orthotopic animal model.
Fichier principal
Manuscript.pdf (306.87 Ko)
Télécharger le fichier
Figure 1.tif (1.14 Mo)
Télécharger le fichier
Figure 2.tif (1.56 Mo)
Télécharger le fichier
Figure 3.tif (365 Ko)
Télécharger le fichier
Figure 4.tif (500.85 Ko)
Télécharger le fichier
Figure 5.tif (4.21 Mo)
Télécharger le fichier
Figure 6.tif (2.18 Mo)
Télécharger le fichier
Figures legend.pdf (74.97 Ko)
Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)