Structural Basis for NusA Stabilized Transcriptional Pausing

Xieyang Guo (1) , Alexander G. Myasnikov (2) , James Chen , Corinne Crucifix (1) , Gabor Papai (1) , Mária Takács (1) , Patrick Schultz (2) , Albert Weixlbaumer (1)

1 IGBMC - Institut de Génétique et de Biologie Moléculaire et Cellulaire
2 IGBMC - Institut de génétique et biologie moléculaire et cellulaire

Highlights d Two cryo-EM reconstructions of paused RNAP elongation complexes bound by NusA d NusA provides positively charged cavity for RNA structures and stabilizes pause d Asymmetric translocation intermediate explains transcriptional pausing d Dynamic process of pausing reflected by RNAP global conformational changes

Domaines: Biologie structurale [q-bio.BM]

Xieyang Guo, Alexander G. Myasnikov, James Chen, Corinne Crucifix, Gabor Papai, et al.. Structural Basis for NusA Stabilized Transcriptional Pausing. Molecular Cell, 2018, 69 (5), pp.816-827.e4. ⟨10.1016/j.molcel.2018.02.008⟩. ⟨hal-02342277⟩