Evaluation of the DiversiLab® automated repetitive sequence-based PCR system for the characterization of Candida albicans and Candida glabrata isolates.

M. Sasso (1) , A.-C. Normand , A. Pantel (2, 1) , N. Bourgeois (3) , L. Lachaud (4, 1)

1 CHU Nîmes - Centre Hospitalier Universitaire de Nîmes
2 VBMI - Virulence bactérienne et maladies infectieuses
3 CHRU Montpellier - Centre Hospitalier Régional Universitaire [Montpellier]
4 UM - Université de Montpellier

The objective of our study was to assess the DiversiLab\textregistered automated repetitive sequence-based PCR (rep-PCR) system for typing C.~albicans and C.~glabrata isolates and to compare it with two proven and routinely used typing methods. A total of 39 isolates from 11 patients with candidaemia or tissue candidiasis (two to six isolates per patient) were analyzed with three typing methods: DiversiLab\textregistered rep-PCR, multilocus sequence typing and multilocus microsatellite typing. DiversiLab\textregistered rep-PCR results were consistent with those obtained using the two other typing methods for C.~albicans, but not for C.~glabrata. Thanks to its simplicity of use, rapidity, standardization and reproducibility, the DiversiLab\textregistered rep-PCR system is an interesting tool to investigate C.~albicans infections.

Mots clés: Humans; Reproducibility of Results; Polymerase Chain Reaction; Automation; Candida albicans; Genotype; Candida glabrata; Microsatellite Repeats; Candida~albicans; Candida~glabrata; Candidiasis; DiversiLab(\textregistered); MultiLocus microsatellite typing; MultiLocus sequence typing; Mycological Typing Techniques; Rep-PCR; Repetitive Sequences; Nucleic Acid; Typing method

Domaines: Sciences du Vivant [q-bio]

M. Sasso, A.-C. Normand, A. Pantel, N. Bourgeois, L. Lachaud. Evaluation of the DiversiLab® automated repetitive sequence-based PCR system for the characterization of Candida albicans and Candida glabrata isolates.. Journal of Medical Mycology = Journal de Mycologie Médicale, 2018, 28 (2), pp.320--326. ⟨10.1016/j.mycmed.2018.03.003⟩. ⟨hal-02341134⟩