Biologically active lipid A antagonist embedded in a multilayered polyelectrolyte architecture

Sophie C. Gangloff (1) , Guy Ladam (2) , Kochi Fukase , Klaus Brandenburg (3) , Moncef Guenounou (1) , Pierre Schaaf (4) , Jean-Claude Voegel (5) , Nadia Jessel (6) , Valérie Dupray (7)

1 URCA - Université de Reims Champagne-Ardenne
2 La2B - Laboratoire de Biophysique et Biomatériaux
3 Biophysik
4 ICS - Institut Charles Sadron
5 Biomatériaux : Processus biophysiques et biologiques aux interfaces
6 UNISTRA - Université de Strasbourg
7 SMS - Sciences et Méthodes Séparatives

Recently [Jessel N, Schwinte P, Donohue R, Lavalle P, Boulmedais F, Darcy R, et al. Pyridylamino-b-cyclodextrin as a molecular chaperone for lipopolysaccharide embedded in a multilayered polyelectrolyte architecture. Adv Funct Mater 2004;14:963-9], we demonstrated the biological activity of a lipopolysaccharide from Escherichia coli incorporated into layer-by-layer films made of poly (L-lysine) and poly (L-glutamic acid) and containing a polycationic b-cyclodextrin (CD) with chaperone properties. Here we develop innovative architectures containing a complex made of a charged b-cyclodextrin and a lipid A antagonist (LAA) as potential systems for local endotoxin antagonistic activity. We examine the biological activity of these architectures. The CD-LAA complex adsorbed on top, or embedded into the polyelectrolyte films keeps its LPS antagonistic activity on both murine and human macrophages for at least 24 h. r

Mots clés: Layer-by-layer films; Anti-inflammatory activity; Lipid A antagonist; Cyclodextrin

Domaines: Biomatériaux; Polymères; Chimie théorique et/ou physique; Chimie thérapeutique

Sophie C. Gangloff, Guy Ladam, Kochi Fukase, Klaus Brandenburg, Moncef Guenounou, et al.. Biologically active lipid A antagonist embedded in a multilayered polyelectrolyte architecture. Biomaterials, 2006, 27 (9), pp.1771-1777. ⟨10.1016/j.biomaterials.2005.10.001⟩. ⟨hal-02338026⟩