Conventional Matrices Loaded Onto a Graphene Layer Enhances MALDI-TOF/TOF Signal: Its Application to Improve Detection of Phosphorylated Peptides

Carlos Rodriguez (1) , Javier Palacios (1) , Ignacio Fajardo (1, 2) , José Luis Urdiales (1, 2) , Xavier Le Guével (3) , Jose Lozano (1) , Francisca Sánchez-Jiménez (1, 2)

1 Universidad de Málaga [Málaga] = University of Málaga [Málaga]
2 CIBER de Enfermedades Raras (CIBERER)
3 Cancer Targets & Experimental Therapeutics

This is the first study where graphene is used as a MALDI adjuvant in combination with the traditional matrix α-cyano-4-hydroxycinnamic acid (CHCA) to improve the signal intensity of peptide samples. Use of this amended matrix not only leads to increased signals but also to a higher number of peaks detected in complex samples. Additionally, the use of graphene has a stabilizing effect that can also be exploited to improve the detection of easily cleavable molecules.

Mots clés: Phosphorylated peptides; MALDI-TOF/TOF; Graphene

Domaines: Chimie analytique

Carlos Rodriguez, Javier Palacios, Ignacio Fajardo, José Luis Urdiales, Xavier Le Guével, et al.. Conventional Matrices Loaded Onto a Graphene Layer Enhances MALDI-TOF/TOF Signal: Its Application to Improve Detection of Phosphorylated Peptides. Journal of The American Society for Mass Spectrometry, 2016, 27 (2), pp.366-369. ⟨10.1007/s13361-015-1299-3⟩. ⟨hal-02338004⟩