Design and Synthesis of Hybrid PEGylated Metal Monopicolinate Cyclam Ligands for Biomedical Applications

Fatima Aouidat (1) , Zakaria Halime (2) , Rosalba Moretta (3) , Ilaria Rea (3) , Stefania Filosa (4) , Stella Donato (5) , Rosarita Tatè (6) , Luca de Stefano (3) , Raphaël Tripier (2) , Jolanda Spadavecchia (1)

1 CSPBAT - Chimie, Structures et Propriétés de Biomatériaux et d'Agents Thérapeutiques
2 CEMCA - Chimie, Electrochimie Moléculaires et Chimie Analytique
3 Institute for Microelectronics and Microsystems [Naples]
4 Institute of Genetics and Biophysics
5 Institute of Biosciences and Bioresources
6 IGB - Institute of Genetics and Biophysics Adriano Buzzati-Traverso

In this study, we report, for the first time, the synthesis of two original nanosystems, based on gold Au(III) and copper Cu(II): simple gold−copper nanoparticles (Cu 0 AuNPs) and enriched monopicolinate cyclam (L1)−Cu(II)−Au(III)-complex (L1@Cu 2+ AuNPs). The two nanomaterials differ substantially by the chelation or not of the Cu(II) ions during the NPs synthesis process. The two hybrid nanoparticles (Cu 0 AuNPs; L1@Cu 2+ AuNPs) were deeply studied from the chemical and physical point of view, using many different analytical techniques such as Raman and UV−vis spectroscopy, electron transmission microscopy, and dynamic light scattering. Both nanosystems show morphological and good chemical stability at pH 4 values and in physiological conditions during 98 h. Undifferentiated and neural differentiated murine embryonic stem cells were used as a model system for in vitro experiments to reveal the effects of NPs on these cells. The comparative study between Cu 0 AuNPs and L1@Cu 2+ AuNPs highlights that copper chelated in its +2 oxidation state in the NPs is more functional for biological application.

Domaines: Chimie; Matériaux

Fatima Aouidat, Zakaria Halime, Rosalba Moretta, Ilaria Rea, Stefania Filosa, et al.. Design and Synthesis of Hybrid PEGylated Metal Monopicolinate Cyclam Ligands for Biomedical Applications. ACS Omega, 2019, 4 (2), pp.2500-2509. ⟨10.1021/acsomega.8b03266⟩. ⟨hal-02337561⟩