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, Linear regression analysis of TAM and tumor volumes
, , p.26
, Total RNA was extracted from MDA-MB-231 tumor xenografts at the end of treatment, and CD206 expression analyzed by RT-qPCR and shown relative to F4/80 (n=9 for CTRL
, for F1 versus CTRL; P=0.04 for F1Fc versus CTRL (Student's t-test)
The percentage of Gr1 + CD11b + MDSCs was quantified by FACS analysis and expressed relative to all CD45 + cells (n=9 for CTRL; n=9 for F1 ,
008 for F1 versus CTRL; P=0.079 for F1Fc versus CTRL (Student's t-test) ,
, Linear regression analysis of MDSC and tumor volumes
, Total RNA was extracted from MDA-MB-231 tumor cell xenografts at the end of treatment and TGF? expression analyzed by RT-qPCR. Data are relative to RPS9 expression (n=9 for CTRL
009 for F1 versus CTRL; P=0.1 for F1Fc versus CTRL (Student's t-test) ,
The percentage of CD49b + CD11b + NK cells was quantified by FACS and expressed relative to all CD45 + cells (mean ± SEM; n=9 for rituximab (CTRL); n=9 for F1 ,
, 7 for F1 versus CTRL
, for F1Fc versus CTRL, vol.8
, 8 for F1 versus F1Fc (Student's t-test)
, Total RNA was extracted from MDA-MB-231 tumor cell xenografts at the end of treatment and IL-15 analyzed by RT-qPCR. Data are the mean ± SEM expression level relative to RPS9 expression (n=9 for rituximab (CTRL); n=9 for F1; n=8 for F1Fc. **, P=0.0013 for F1 versus CTRL
0127 for F1 versus F1Fc (Student's t-test) ,
, Linear regression analysis of IL-15 mRNA level and tumor volumes. R 2 =0.3693
, , p.26
, Quantification of granzyme B mRNA expression as in (B)
0002 for F1 versus CTRL ,
0011 for F1Fc versus CTRL ,
0076 for F1 versus F1Fc (Student's t-test) ,
, Quantification of perforin mRNA expression as in (B). *, P=0.033 for F1 versus CTRL; *, P=0.0294 for F1Fc versus CTRL; P=0.386 for F1 versus F1Fc
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