ABC-F proteins in mRNA translation and antibiotic resistance

Fares Ousalem (1) , Shikha Singh (2) , Olivier Chesneau (3) , John Hunt (2) , Grégory Boël (1)

1 EGM (UMR_8261 / FRE_3630) - Expression Génétique Microbienne
2 Columbia University [New York]
3 Département de Microbiologie - Department of Microbiology

The ATP binding cassette protein superfamily comprises ATPase enzymes which are, for the most part, involved in transmembrane transport. Within this superfamily however, some protein families have other functions unrelated to transport. One example is the ABC-F family, which comprises an extremely diverse set of cytoplasmic proteins. All of the proteins in the ABC-F family characterized to date act on the ribosome and are translation factors. Their common function is ATP-dependent modulation of the stereochemistry of the peptidyl transferase center (PTC) in the ribosome coupled to changes in its global conformation and P-site tRNA binding geometry. In this review, we give an overview of the function, structure, and theories for the mechanisms-of-action of microbial proteins in the ABC-F family, including those involved in mediating resistance to ribosome-binding antibiotics.

Mots clés: Antibiotic resistance; ATPase; ABC-F protein family; mRNA translation; Translation regulation; ABC superfamily; translation regulation; antibiotic resistance

Domaines: Biochimie, Biologie Moléculaire

Fares Ousalem, Shikha Singh, Olivier Chesneau, John Hunt, Grégory Boël. ABC-F proteins in mRNA translation and antibiotic resistance. Research in Microbiology, 2019, 170 (8), pp.435-447. ⟨10.1016/j.resmic.2019.09.005⟩. ⟨hal-02324918⟩