Analysis of 5000 year-old human teeth using optimized large-scale and targeted proteomics approaches for detection of sex-specific peptides

Carine Froment (1) , Mathilde Hourset (2) , Nancy Sáenz-Oyhéréguy (2) , Emmanuelle Mouton-Barbosa (1) , Claire Willmann (2) , Clément Zanolli (2) , Rémi Esclassan (3) , Richard Donat (2) , Catherine Thèves (2) , Odile Burlet-Schiltz (1) , Catherine Mollereau (1)

1 IPBS - Institut de pharmacologie et de biologie structurale
2 AMIS - Anthropologie Moléculaire et Imagerie de Synthèse
3 UT3 - Université Toulouse III - Paul Sabatier

The study demonstrates the high potential of MS-based proteomics coupled to an iterative database search strategy for the in-depth investigation of ancient proteomes. An efficient targeted PRM MS-based approach, although limited to the detection of a single pair of sex-specific amelogenin peptides, allowed confirming the sex of individuals in ancient dental remains, an essential information for paleoanthropologists facing the issue of sex determination and dimorphism.

Mots clés: Paleoproteomics; Data dependent acquisition; Parallel reaction monitoring; Tooth; Amelogenin; Sex determination

Domaines: Sciences du Vivant [q-bio]; Sciences de l'Homme et Société

Carine Froment, Mathilde Hourset, Nancy Sáenz-Oyhéréguy, Emmanuelle Mouton-Barbosa, Claire Willmann, et al.. Analysis of 5000 year-old human teeth using optimized large-scale and targeted proteomics approaches for detection of sex-specific peptides. Journal of Proteomics, 2020, 211, pp.103548. ⟨10.1016/j.jprot.2019.103548⟩. ⟨hal-02322441⟩