Nanoscale organization of tetraspanins during HIV-1 budding by correlative dSTORM/AFM

Selma Dahmane (1) , Christine Doucet (1) , Antoine Le Gall (1) , Célia Chamontin , Patrice Dosset (1) , Florent Murcy (1) , Laurent Fernandez (1) , Desiree Salas (1) , Eric Rubinstein (2) , Marylène Mougel , Marcelo Nollmann (1) , Pierre-Emmanuel Milhiet (1)

1 CBS - Centre de Biochimie Structurale [Montpellier]
2 CIMI - Centre d'Immunologie et des Maladies Infectieuses

Membrane partition and remodeling play a key role in numerous cell mechanisms, especially in viral replication cycles where viruses subvert the plasma membrane to enter and escape from the host cell. Specifically assembly and release of HIV-1 particles require specific cellular components, which are recruited to the egress site by the viral protein Gag. We previously demonstrated that HIV-1 assembly alters both partitioning and dynamics of the tetraspanins CD9 and CD81, which are key players in many infectious processes, forming enriched areas where the virus buds. In this study we correlated super resolution microscopy mapping of tetraspanins with membrane topography delineated by atomic force microscopy (AFM) in Gag-expressing cells. We revealed that CD9 is specifically trapped within the nascent viral particles, especially at buds tips, suggesting that Gag mediates CD9 and CD81 depletion from the plasma membrane. In addition, we showed that CD9 is organized as small membrane assemblies of few tens of nanometers that can coalesce upon Gag expression. † Electronic supplementary information (ESI) available. See

Domaines: Sciences du Vivant [q-bio]

Selma Dahmane, Christine Doucet, Antoine Le Gall, Célia Chamontin, Patrice Dosset, et al.. Nanoscale organization of tetraspanins during HIV-1 budding by correlative dSTORM/AFM. Nanoscale, 2019, 11, pp.6036 - 6044. ⟨10.1039/c8nr07269h⟩. ⟨hal-03103990v2⟩