IL-10 Producing B Cells Ability to Induce Regulatory T Cells Is Maintained in Rheumatoid Arthritis

Julie Mielle (1, 2) , Rachel Audo (1, 2) , Michael Hahne (1) , Laurence Macia (3) , Bernard Combe (2, 1) , Jacques Morel (2, 1) , Claire Daien (2, 1)

1 IGMM - Institut de Génétique Moléculaire de Montpellier
2 Département de Rhumatologie[Montpellier]
3 The University of Sydney

Despite growing evidence highlighting the relevance of increasing IL-10-producing B cells (B10+cells) in autoimmune diseases, their functions in patients are still unknown. The aim of this study was to evaluate the functions of CpG-induced B10+ cells isolated from healthy controls (HC) and rheumatoid arthritis (RA) patients, on naïve T cell differentiation. We demonstrated that CpG-induced B10+ cells from HC drove naïve T cell differentiation toward regulatory T cells (Treg cells) and IL-10-producing T cells (Tr1) through IL-10 secretion and cellular contacts. B10+ cells from HC did not decrease T helper 1 (Th1) nor and tumor necrosis factor α producing T cell (TNFα+ T cell) differentiation. We showed that in RA, B10+ cells could also induce Treg cells and Tr1 from naïve T cells. Contrary to HC, B10+ cells from RA patients increased naïve T cell conversion into Th1. Interestingly, PD-L2, a programmed death-1 (PD-1) ligand that inhibits PD-L1 and promotes Th1 differentiation, was overexpressed on RA B10+ cells compared to HC B10+ cells. Together, our findings showed that CpG-induced B10+ cells may be used to increase Treg cells in patients with RA. However, CpG may not be the most adequate stimuli as CpG-induced B10+ cells also increased inflammatory T cells in those patients.

Mots clés: regulatory B cell; autoimmunity; interleukin 10; programmed death-ligand 2; rheumatoid arthritis; B10

Domaines: Rhumatologie et système ostéo-articulaire; Immunité adaptative

Julie Mielle, Rachel Audo, Michael Hahne, Laurence Macia, Bernard Combe, et al.. IL-10 Producing B Cells Ability to Induce Regulatory T Cells Is Maintained in Rheumatoid Arthritis. Frontiers in Immunology, 2018, 9, pp.961. ⟨10.3389/fimmu.2018.00961⟩. ⟨hal-02320501⟩