Staphylococcus pseudoxylosus sp. nov., isolated from bovine mastitis

A. Macfadyen (1) , Sabine Leroy (2) , Elaine Harrison (3) , Martin Holmes (4) , Kenneth G. Paterson (1)

1 Edin. - University of Edinburgh
2 MEDIS - Microbiologie Environnement Digestif Santé
3 The Wellcome Trust Sanger Institute [Cambridge]
4 CAM - University of Cambridge [UK]

Strain S04009T, a Gram-stain-positive, coagulase-negative staphylococcus, was isolated from bovine mastitis in France. 16S rRNA gene analysis revealed it to be closely related to the coagulase-negative species Staphylococcus xylosus , Staphylococcus saprophyticus , Staphylococcus caeli and Staphylococcus edaphicus . At the whole-genome level, strain S04009T had an average nucleotide identity value <95 % and an inferred DNA–DNA hybridization value <70 % when compared to these species. Furthermore, phenotypic characteristics distinguished S04009T from those species. From these related species only strain S04009T and S. xylosus are able to ferment xylose and these two can be distinguished by the inability of strain S04009T to express urease activity. Based on the genotypic and phenotypic results, it is proposed that this isolate is a novel species, with the name Staphylococcus pseudoxylosus sp. nov. The type strain is S04009T (=DSM 107950T=CCUG 72763T=NCTC 14184T).

Mots clés: mastitis; mecC; Staphylococcus xylosus and Staphylococcus; Staphyloccus pseudoxylosus

Domaines: Bactériologie; Systématique, phylogénie et taxonomie

A. Macfadyen, Sabine Leroy, Elaine Harrison, Martin Holmes, Kenneth G. Paterson. Staphylococcus pseudoxylosus sp. nov., isolated from bovine mastitis. International Journal of Systematic and Evolutionary Microbiology, 2019, 69 (8), pp.003416. ⟨10.1099/ijsem.0.003416⟩. ⟨hal-02320288⟩