Skip to Main content Skip to Navigation
Journal articles

Pseudomonas aeruginosa Transmigrates at Epithelial Cell-Cell Junctions, Exploiting Sites of Cell Division and Senescent Cell Extrusion

Guillaume Golovkine 1 Eric Faudry 1 Stéphanie Bouillot 1 Sylvie Elsen 1 Ina Attree 1 Philippe Huber 1
1 PBRC - Pathogenèse bactérienne et réponses cellulaires
BCI - Biologie du Cancer et de l'Infection, CNRS - Centre National de la Recherche Scientifique : ERL 526
Abstract : To achieve systemic infection, bacterial pathogens must overcome the critical and challenging step of transmigration across epithelial barriers. This is particularly true for opportunistic pathogens such as Pseudomonas aeruginosa, an agent which causes nosocomial infections. Despite extensive study, details on the mechanisms used by this bacterium to transmigrate across epithelial tissues, as well as the entry sites it uses, remain speculative. Here, using real-time microscopy and a model epithelial barrier, we show that P. aeruginosa employs a paracellular transmigration route, taking advantage of altered cell-cell junctions at sites of cell division or when senescent cells are expelled from the cell layer. Once a bacterium transmigrates, it is followed by a cohort of bacteria using the same entry point. The basal compartment is then invaded radially from the initial penetration site. Effective transmigration and propagation require type 4 pili, the type 3 secretion system (T3SS) and a flagellum, although flagellum-deficient bacteria can occasionally invade the basal compartment from wounded areas. In the basal compartment, the bacteria inject the T3SS toxins into host cells, disrupting the cytoskeleton and focal contacts to allow their progression under the cells. Thus, P. aeruginosa exploits intrinsic host cell processes to breach the epithelium and invade the subcellular compartment.
Complete list of metadatas

https://hal.archives-ouvertes.fr/hal-02318956
Contributor : Philippe Huber <>
Submitted on : Thursday, October 17, 2019 - 3:27:19 PM
Last modification on : Thursday, March 26, 2020 - 6:57:45 PM

Links full text

Identifiers

Collections

Citation

Guillaume Golovkine, Eric Faudry, Stéphanie Bouillot, Sylvie Elsen, Ina Attree, et al.. Pseudomonas aeruginosa Transmigrates at Epithelial Cell-Cell Junctions, Exploiting Sites of Cell Division and Senescent Cell Extrusion. PLoS Pathogens, Public Library of Science, 2016, 12 (1), pp.e1005377. ⟨10.1371/journal.ppat.1005377⟩. ⟨hal-02318956⟩

Share

Metrics

Record views

206