First access to a mycolic acid-based bioorthogonal reporter for the study of the mycomembrane and mycoloyltransferases in Corynebacteria

Emilie Lesur , Aurélie Baron , Christiane Dietrich , Marie Buchotte , Gilles Doisneau , Dominique Urban , Jean-Marie Beau , Nicolas Bayan (1, 2) , Boris Vauzeilles , Dominique Guianvarc'H , Yann Bourdreux

1 I2BC - Institut de Biologie Intégrative de la Cellule
2 CORYNE - Biologie Moléculaire des Corynébactéries et Mycobactéries

In this study, we report the first synthesis of an alkyne-based trehalose monomycolate probe containing a β-hydroxylated fatty acid and an α-branched chain similar to those of the natural mycolic acid. We demonstrate its utility for the labeling of the mycomembrane of Corynebacteria as well as for the study of mycoloyltransferases.

Domaines: Sciences du Vivant [q-bio]

Emilie Lesur, Aurélie Baron, Christiane Dietrich, Marie Buchotte, Gilles Doisneau, et al.. First access to a mycolic acid-based bioorthogonal reporter for the study of the mycomembrane and mycoloyltransferases in Corynebacteria. Chemical Communications, 2019, ⟨10.1039/c9cc05754d⟩. ⟨hal-02318404⟩