An Enteral Leucine Supply Modulates Human Duodenal Mucosal Proteome and Decreases the Expression of Enzymes Involved in Fatty Acid Beta-Oxidation

Alexis Goichon (1, 2, 3) , Philippe Chan (4, 2, 3) , Stéphane Lecleire (5, 1, 2, 3) , Aude Coquard (6) , Anne-Françoise Cailleux (7, 2, 3) , Stéphane Walrand (8) , Eric Lerebours (5, 2, 3, 1) , David Vaudry (9, 2, 3) , Pierre Déchelotte (1, 2, 3, 10) , Moïse Coëffier (1, 2, 3, 10)

1 ADEN - Nutrition, inflammation et dysfonctionnement de l'axe intestin-cerveau
2 UNIROUEN - Université de Rouen Normandie
3 IRIB - Institute for Research and Innovation in Biomedicine
4 Plate-forme de Protéomique PISSARO
5 Service d'Hépato-Gastroentérologie [CHU Rouen]
6 Pharmacie [CHU Rouen]
7 CIC Rouen - Centre d'Investigation Clinique [CHU Rouen]
8 UNH - Unité de Nutrition Humaine
9 DC2N - Différenciation et communication neuronale et neuroendocrine
10 Service de Nutrition [CHU Rouen]

Leucine is well known to regulate protein metabolism in muscle. We recently reported that enteral leucine infusion decreased proteasome activity in human duodenal mucosa and enhanced intestinal cell proliferation, but its effects on gut proteome remain unknown. Therefore, we aimed to assess the effects of an enteral leucine infusion on the whole proteome of duodenal mucosa. In this work, 5 healthy volunteers received for 5h, on 2 occasions and in random order, an enteral supply of maltodextrins (0.25 g kg(-1) h(-1)) or maltodextrins supplemented with leucine (0.035 g kg(-1) h(-1)). At the end of infusion, endoscopic duodenal biopsy samples were collected and analyzed by 2D-PAGE. Eleven protein spots were differentially and significantly (P<0.05) expressed in response to the leucine-supplemented maltodextrins compared with maltodextrins alone. Forty percent of identified proteins by mass spectrometry were located in mitochondria. Four proteins were involved in lipid metabolism: HADHA, ACADVL and CPT2 expressions were reduced, whereas FABP1 expression was increased. In addition, the expression of DHA kinase involved in glycerol metabolism was also downregulated. Finally, leucine supplementation altered the duodenal mucosal proteome by regulating the expression of several enzymes mainly involved in lipid metabolism. These results suggest that leucine supplementation may slowdown fatty acid beta-oxidation in human duodenal mucosa.

Mots clés: Adolescent; Adult; Duodenum; Electrophoresis; Gel; Two-Dimensional; Fatty Acids; Female; Humans; Intestinal Mucosa; Leucine; Lipid Metabolism; Male; Oxidation-Reduction; Proteome

Domaines: Alimentation et Nutrition

Alexis Goichon, Philippe Chan, Stéphane Lecleire, Aude Coquard, Anne-Françoise Cailleux, et al.. An Enteral Leucine Supply Modulates Human Duodenal Mucosal Proteome and Decreases the Expression of Enzymes Involved in Fatty Acid Beta-Oxidation. Journal of Proteomics, 2013, 78, pp.535-544. ⟨10.1016/j.jprot.2012.10.024⟩. ⟨hal-02305179⟩