Skip to Main content Skip to Navigation

Experimental Approaches to Study Genome Packaging of Influenza A Viruses

Abstract : The genome of influenza A viruses (IAV) consists of eight single-stranded negative sense viral RNAs (vRNAs) encapsidated into viral ribonucleoproteins (vRNPs). It is now well established that genome packaging (i.e., the incorporation of a set of eight distinct vRNPs into budding viral particles), follows a specific pathway guided by segment-specific cis-acting packaging signals on each vRNA. However, the precise nature and function of the packaging signals, and the mechanisms underlying the assembly of vRNPs into sub-bundles in the cytoplasm and their selective packaging at the viral budding site, remain largely unknown. Here, we review the diverse and complementary methods currently being used to elucidate these aspects of the viral cycle. They range from conventional and competitive reverse genetics, single molecule imaging of vRNPs by fluorescence in situ hybridization (FISH) and high-resolution electron microscopy and tomography of budding viral particles, to solely in vitro approaches to investigate vRNA-vRNA interactions at the molecular level.
Document type :
Journal articles
Complete list of metadatas

Cited literature [62 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-02294220
Contributor : Sandra Mathelin <>
Submitted on : Wednesday, October 2, 2019 - 6:19:10 PM
Last modification on : Friday, March 27, 2020 - 3:02:46 AM

File

viruses-08-00218.pdf
Publication funded by an institution

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Catherine Isel, Sandie Munier, Nadia Naffakh. Experimental Approaches to Study Genome Packaging of Influenza A Viruses. Viruses, MDPI, 2016, 8 (8), pp.218. ⟨10.3390/v8080218⟩. ⟨hal-02294220⟩

Share

Metrics

Record views

198

Files downloads

211