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Article Dans Une Revue Nature Communications Année : 2014

BIN1/M-Amphiphysin2 induces clustering of phosphoinositides to recruit its downstream partner dynamin

Laura Picas
Stefano Vanni
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Karim Hnia
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Aurelien Roux
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Patricia Bassereau
Bruno Goud

Résumé

Phosphoinositides play a central role in many physiological processes by assisting the recruitment of proteins to membranes through specific phosphoinositide-binding motifs. How this recruitment is coordinated in space and time is not well understood. Here we show that BIN1/M-Amphiphysin2, a protein involved in T-tubule biogenesis in muscle cells and frequently mutated in centronuclear myopathies, clusters PtdIns(4,5)P2 to recruit its downstream partner dynamin. By using several mutants associated with centronuclear myopathies, we find that the N-BAR and the SH3 domains of BIN1 control the kinetics and the accumulation of dynamin on membranes, respectively. We show that phosphoinositide clustering is a mechanism shared by other proteins that interact with PtdIns(4,5)P2, but do not contain a BAR domain. Our numerical simulations point out that clustering is a diffusion-driven process in which phosphoinositide molecules are not sequestered. We propose that this mechanism plays a key role in the recruitment of downstream phosphoinositide-binding proteins.

Dates et versions

hal-02289097 , version 1 (16-09-2019)

Identifiants

Citer

Laura Picas, Julien Viaud, Kristine Schauer, Stefano Vanni, Karim Hnia, et al.. BIN1/M-Amphiphysin2 induces clustering of phosphoinositides to recruit its downstream partner dynamin. Nature Communications, 2014, 5 (1), pp.5647. ⟨10.1038/ncomms6647⟩. ⟨hal-02289097⟩
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