Functional analysis of isoprenoid precursors biosynthesis by quantitative metabolomics and isotopologue profiling

Sara Castaño-Cerezo (1) , Hanna Kulyk-Barbier (1, 2) , Pierre Millard (1) , Jean-Charles Portais (1, 2) , Stephanie Heux (1) , Gilles Truan (1) , Floriant Bellvert (1, 2)

1 LISBP - Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés
2 E20 - MetaToul AXIOM

Isoprenoids are amongst the most abundant and diverse biological molecules and are involved in a broad range of biological functions. Functional understanding of their biosynthesis is thus key in many fundamental and applicative fields, including systems biology, medicine and biotechnology. However, available methods do not yet allow accurate quantification and tracing of stable isotopes incorporation for all the isoprenoids precursors.

Mots clés: Systems biology; Mass spectrometry; Isoprenoids; Isotope labeling experiments

Domaines: Biotechnologies; Chimie analytique

Sara Castaño-Cerezo, Hanna Kulyk-Barbier, Pierre Millard, Jean-Charles Portais, Stephanie Heux, et al.. Functional analysis of isoprenoid precursors biosynthesis by quantitative metabolomics and isotopologue profiling. Metabolomics, 2019, 15 (9), 10 p. ⟨10.1007/s11306-019-1580-8⟩. ⟨hal-02269068⟩