Automatic Registration of Correlative Microscopies with Error Assessment and Applications for the Optimization of Multimodal Acquisitions.

Guillaume Potier (1) , Romain Capoulade (1) , Jean Mérot (1) , Frédéric Lavancier (2) , Xavier Heiligenstein (3) , Jean Salamero (4) , Perrine Paul-Gilloteaux (5, 1)

1 ITX - ITX - unité de recherche de l'institut du thorax
2 SERPICO - Space-timE RePresentation, Imaging and cellular dynamics of molecular COmplexes
3 CDC - Compartimentation et dynamique cellulaires
4 PICT-IBiSA - BioImaging Cell and Tissue Core Facility
5 SFR François Bonamy - Structure fédérative de recherche François Bonamy

Correlative microscopy allows combining different scales of observations and different contents, functional and morphological, based on the large panel of microscopy technologies available for life or material sciences. The purpose is to accurately follow a region of interest across modalities and scales of imaging, for guiding and targeting the specimen preparation procedure needed for another modality, to guide the acquisition at the second microscope and to adjust the field of view of the microscope to this feature. In a last, and most accurate registration step, the acquired data from both modalities are mapped.

Domaines: Traitement des images [eess.IV]; Imagerie; Statistiques [math.ST]; Traitement du signal et de l'image [eess.SP]

Guillaume Potier, Romain Capoulade, Jean Mérot, Frédéric Lavancier, Xavier Heiligenstein, et al.. Automatic Registration of Correlative Microscopies with Error Assessment and Applications for the Optimization of Multimodal Acquisitions.. Microscopy and Microanalysis, 2019, 25 (S2), pp.1020-1021. ⟨10.1017/S143192761900583X⟩. ⟨hal-02267554⟩