Cdh2 coordinates Myosin-II dependent internalisation of the zebrafish neural plate

Abstract : Tissue internalisation is a key morphogenetic mechanism by which embryonic tissues generate complex internal organs and a number of studies of epithelia have outlined a general view of tissue internalisation. Here we have used quantitative live imaging and mutant analysis to determine whether similar mechanisms are responsible for internalisation in a tissue that apparently does not have a typical epithelial organisation - the zebrafish neural plate. We found that although zebrafish embryos begin neurulation without a conventional epithelium, medially located neural plate cells adopt strategies typical of epithelia in order to constrict their dorsal surface membrane during cell internalisation. Furthermore, we show that Myosin-II activity is a significant driver of this transient cell remodeling which also depends on Cdh2 (N-cadherin). Abrogation of Cdh2 results in defective Myosin-II distribution, mislocalised internalisation events and defective neural plate morphogenesis. Our work suggests Cdh2 coordinates Myosin-II dependent internalisation of the zebrafish neural plate.
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https://hal.archives-ouvertes.fr/hal-02265282
Contributor : Alain Perignon <>
Submitted on : Friday, August 9, 2019 - 10:55:50 AM
Last modification on : Saturday, August 10, 2019 - 1:07:35 AM

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Claudio Araya, Hanna-Maria Häkkinen, Luis Carcamo, Mauricio Cerda, Thierry Savy, et al.. Cdh2 coordinates Myosin-II dependent internalisation of the zebrafish neural plate. Scientific Reports, Nature Publishing Group, 2019, 9 (1), pp.1835. ⟨10.1038/s41598-018-38455-w⟩. ⟨hal-02265282⟩

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