DNA arms do the legwork to ensure the directionality of lambda site-specific recombination

Abstract : The integrase protein of bacteriophage lambda (Int) catalyzes site-specific recombination between lambda phage and Escherichia coli genomes. Int is a tyrosine recombinase that binds to DNA core sites via a C-terminal catalytic domain and to a collection of arm DNA sites, distant from the site of recombination, via its N-terminal domain. The arm sites, in conjunction with accessory DNA-bending proteins, provide a means of regulating the efficiency and directionality of Int-catalyzed recombination. Recent crystal structures of lambda Int tetramers bound to synaptic and Holliday junction intermediates, together with new biochemical data, suggest a mechanism for the allosteric control of the recombination reaction through arm DNA binding interactions.
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Submitted on : Friday, August 2, 2019 - 3:19:17 PM
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M. Radman-Livaja, T. Biswas, T. Ellenberger, A. Landy, H. Aihara. DNA arms do the legwork to ensure the directionality of lambda site-specific recombination. Current Opinion in Structural Biology, 2006, 16 (1), pp.42--50. ⟨10.1016/j.sbi.2005.12.003⟩. ⟨hal-02262469⟩

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