A map of the interactome network of the metazoan C-elegans

S. M. Li (1) , C. M. Armstrong , N. Bertin , H. Ge , S. Milstein , M. Boxem , P. O. Vidalain , J. D. J. Han , A. Chesneau (2) , T. Hao , D. S. Goldberg , N. Li , M. Martinez (3) , J. F. Rual , P. Lamesch , L. Xu , M. Tewari , S. L. Wong , L. V. Zhang , G. F. Berriz , L. Jacotot (4) , P. Vaglio (4) , J. Reboul , T. Hirozane-Kishikawa , Q. R. Li (5, 6) , H. W. Gabel , A. Elewa , B. Baumgartner , D. J. Rose , H. Y. Yu , S. Bosak , R. Sequerra , A. Fraser , S. E. Mango , W. M. Saxton , S. Strome , S. van Den Heuvel , F. Piano (7) , J. Vandenhaute , C. Sardet (8) , M. Gerstein , L. Doucette-Stamm , K. C. Gunsalus , J. W. Harper , M. E. Cusick , F. P. Roth , D. E. Hill , M. Vidal (9)

1 NILU - Norwegian Institute for Air Research
2 MAOAC - Mécanismes adaptatifs : des organismes aux communautés
3 PennState Meteorology Department
4 Modul-Bio
5 CRISMAT - Laboratoire de cristallographie et sciences des matériaux
6 TU Darmstadt - Technische Universität Darmstadt - Technical University of Darmstadt
7 Department of Biology [York]
8 IGMM - Institut de Génétique Moléculaire de Montpellier
9 IBCP - Institut de biologie et chimie des protéines [Lyon]

To initiate studies on how protein-protein interaction (or "interactome") networks relate to multicellular functions, we have mapped a large fraction of the Caenorhabditis elegans interactome network. Starting with a subset of metazoan-specific proteins, more than 4000 interactions were identified from high-throughput, yeast two-hybrid (HT=Y2H) screens. Independent coaffinity purification assays experimentally validated the overall quality of this Y2H data set. Together with already described Y2H interactions and interologs predicted in silico, the current version of the Worm Interactome (WI5) map contains similar to5500 interactions. Topological and biological features of this interactome network, as well as its integration with phenome and transcriptome data sets, lead to numerous biological hypotheses.

Mots clés: protein-protein interactions caenorhabditis-elegans saccharomyces-cerevisiae gene-expression eye development genome identification information drosophila complexes

Domaines: Biochimie, Biologie Moléculaire

S. M. Li, C. M. Armstrong, N. Bertin, H. Ge, S. Milstein, et al.. A map of the interactome network of the metazoan C-elegans. Science, 2004, 303 (5657), pp.540--543. ⟨10.1126/science.1091403⟩. ⟨hal-02262415⟩