RNase H-Assisted Imaging of Peroxynitrite in Living Cells with 5′-Boronic Acid Modified DNA

Maëva Reverte (1) , Anaïs Vaissière (2) , Prisca Boisguerin (3) , Jean-Jacques Vasseur (1) , Michaël Smietana (1)

1 IBMM - Institut des Biomolécules Max Mousseron [Pôle Chimie Balard]
2 CBS - Centre de Biochimie Structurale [Montpellier]
3 CRBM - Centre de recherche en Biologie cellulaire de Montpellier

RNase H is a ubiquitous intracellular enzyme able to selectively hydrolyze the RNA strand of an RNA/DNA hybrid duplex. In the present study, we demonstrate that 5'-boronic acid modified oligonucleotides could inhibit RNase H activity; actvity which could subsequently be restored upon oxidation of the boronic acid moiety. This unprecedented dual property was exploited to engineer a new stimuli-responsive system for the chemoselective imaging of endogenous and exogenous peroxynitrite in RAW264.7 cells.

Mots clés: NITRATION; FLUORESCENT-PROBE; REACTIVE OXYGEN; NITRIC-OXIDE; SELECTIVE DETECTION; HYDROGEN-PEROXIDE; RIBONUCLEASE-H; FAST-RESPONSE; OXIDATION; STRESS

Domaines: Chimie; Chimie organique; Chimie thérapeutique; Chimie analytique

Maëva Reverte, Anaïs Vaissière, Prisca Boisguerin, Jean-Jacques Vasseur, Michaël Smietana. RNase H-Assisted Imaging of Peroxynitrite in Living Cells with 5′-Boronic Acid Modified DNA. ACS Sensors, 2016, 1 (8), pp.970-974. ⟨10.1021/acssensors.6b00401⟩. ⟨hal-02197251⟩