RNase H-Assisted Imaging of Peroxynitrite in Living Cells with 5′-Boronic Acid Modified DNA

Abstract : RNase H is a ubiquitous intracellular enzyme able to selectively hydrolyze the RNA strand of an RNA/DNA hybrid duplex. In the present study, we demonstrate that 5'-boronic acid modified oligonucleotides could inhibit RNase H activity; actvity which could subsequently be restored upon oxidation of the boronic acid moiety. This unprecedented dual property was exploited to engineer a new stimuli-responsive system for the chemoselective imaging of endogenous and exogenous peroxynitrite in RAW264.7 cells.
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https://hal.archives-ouvertes.fr/hal-02197251
Contributor : Jean-Jacques Vasseur <>
Submitted on : Tuesday, July 30, 2019 - 11:06:21 AM
Last modification on : Wednesday, July 31, 2019 - 1:24:50 AM

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Maëva Reverte, Anaïs Vaissière, Prisca Boisguerin, Jean-Jacques Vasseur, Michaël Smietana. RNase H-Assisted Imaging of Peroxynitrite in Living Cells with 5′-Boronic Acid Modified DNA. ACS Sensors, American Chemical Society, 2016, 1 (8), pp.970-974. ⟨10.1021/acssensors.6b00401⟩. ⟨hal-02197251⟩

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