2-Deoxyribose-5-phosphate aldolase, a remarkably tolerant aldolase towards nucleophile substrates

Abstract : We explored a collection of 2-deoxyribose-5-phosphate aldolases (DERAs) from biodiversity for their nucleophile substrate promiscuity. The DERAs were screened using as nucleophiles propanone, propanal, cyclobutanone, cyclopentanone, dihydroxyacetone, and glycolaldehyde with L-glyceraldehyde-3-phosphate as an electrophile in aldol addition. A DERA from Arthrobacter chlorophenolicus (DERAArthro) efficiently allowed the synthesis of the corresponding aldol adducts in good yields, displaying complementarity in terms of configuration and substrate specificity with fructose-6-phosphate aldolase, the only previously known aldolase with a large nucleophile tolerance.
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https://hal.archives-ouvertes.fr/hal-02195148
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Submitted on : Friday, July 26, 2019 - 11:50:32 AM
Last modification on : Saturday, July 27, 2019 - 1:20:15 AM

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Domitille Chambre, Christine Guérard-Hélaine, Ekaterina Darii, Aline Mariage, Jean-Louis Petit, et al.. 2-Deoxyribose-5-phosphate aldolase, a remarkably tolerant aldolase towards nucleophile substrates. Chemical Communications, Royal Society of Chemistry, 2019, 55 (52), pp.7498-7501. ⟨10.1039/C9CC03361K⟩. ⟨hal-02195148⟩

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