Skip to Main content Skip to Navigation
Journal articles

A novel mouse c-fos intronic promoter that responds to CREB and AP-1 is developmentally regulated in vivo

Abstract : BACKGROUND: The c-fos proto-oncogene is an archetype for rapid and integrative transcriptional activation. Innumerable studies have focused on the canonical promoter, located upstream from the transcriptional start site. However, several regulatory sequences have been found in the first intron. METHODOLOGY/PRINCIPAL FINDINGS: Here we describe an extremely conserved region in c-fos first intron that contains a putative TATA box, and functional TRE and CRE sites. This fragment drives reporter gene activation in fibroblasts, which is enhanced by increasing intracellular calcium and cAMP and by cotransfection of CREB or c-Fos/c-Jun expression vectors. We produced transgenic mice expressing a lacZ reporter controlled by the intronic promoter. Lac Z expression of this promoter is restricted to the developing central nervous system (CNS) and the mesenchyme of developing mammary buds in embryos 12.5 days post-conception, and to brain tissue in adults. RT-QPCR analysis of tissue mRNA, including the anlage of the mammary gland and the CNS, confirms the existence of a novel, nested mRNA initiated in the first intron. CONCLUSIONS/SIGNIFICANCE: Our results provide evidence for a novel, developmentally regulated promoter in the first intron of the c-fos gene.
Document type :
Journal articles
Complete list of metadatas

https://hal.archives-ouvertes.fr/hal-02193432
Contributor : Sarah Adele <>
Submitted on : Wednesday, July 24, 2019 - 2:39:22 PM
Last modification on : Wednesday, August 21, 2019 - 9:38:06 AM

Links full text

Identifiers

Collections

Citation

V. Coulon, K. Chebli, P. Cavelier, J. M. Blanchard. A novel mouse c-fos intronic promoter that responds to CREB and AP-1 is developmentally regulated in vivo. PLoS ONE, Public Library of Science, 2010, 5 (6), pp.e11235. ⟨10.1371/journal.pone.0011235⟩. ⟨hal-02193432⟩

Share

Metrics

Record views

35