A Toolkit for the Characterization of the Photoprotective Capacity of Green Algae

M Águila Ruiz-Sola (1) , Dimitris Petroutsos (1)

1 LPCV - Physiologie cellulaire et végétale

While light is a crucial energy source in photosynthetic organisms, if its intensity exceeds their photosynthetic capacity it may cause light-induced damage. A dominant photoprotective mechanism in plants and algae is the qE (quenching of energy), the major component of nonphotochemical quenching (NPQ). qE is a process that dissipates absorbed excitation energy as heat, ensuring cell survival even under adverse conditions. The present protocol gathers together a set of experimental approaches (in vivo chlorophyll fluorescence, western blotting, growth and cellular chlorophyll content at very strong light) that collectively allow for the characterization of the qE capacity of the model green algae Chlamydomonas reinhardtii.

Mots clés: Nonphotochemical quenching (NPQ); Quenching of energy (qE); Western blotting; High light stress; In vivo chlorophyll fluorescence

Domaines: Biochimie, Biologie Moléculaire

M Águila Ruiz-Sola, Dimitris Petroutsos. A Toolkit for the Characterization of the Photoprotective Capacity of Green Algae. Marechal Eric. Plastids: Methods and Protocols. Series: Methods in Molecular Biology, 1829, Springer, pp.315-323, 2018, ⟨10.1007/978-1-4939-8654-5_21⟩. ⟨hal-02191256⟩