Coupling Electrochemical Adsorption and Long‐range Electron Transfer: Label‐free DNA Mismatch Detection with Ultramicroelectrode (UME)

Nivedita Basu (1, 2) , Thu Huong Ho (1) , François-Xavier Guillon (1) , Yuanyuan Zhang (1) , Pascal Bigey (3) , Bhat Navakanta (2) , Fethi Bedioui (1) , Mathieu Lazerges (1)

1 iCLeHS - Institute of Chemistry for Life and Health Sciences
2 IISc Bangalore - Indian Institute of Science [Bangalore]
3 UTCBS - UM 4 (UMR 8258 / U1022) - Unité de Technologies Chimiques et Biologiques pour la Santé

A new electrochemical hybridization trans-duction pathway, obtained by coupling electrochemical adsorption and long-range electron transfer through double-stranded DNA, was investigated using ultrami-croelectrode (UME). The results show that long-range electron transfer does not occurs exclusively throws well-packed and organized self-assembled DNA monolayers. This approach is used to investigate long-range electron transfer properties of both single-and double-stranded short synthetic DNA and DNA plasmids. Single mismatch electrochemical detection protocol of non-labelled short synthetic DNA, without heating or probe labelling, in a 10 minutes protocol, was in fine performed.

Domaines: Chimie; Chimie analytique; Biotechnologies; Organes des sens

Nivedita Basu, Thu Huong Ho, François-Xavier Guillon, Yuanyuan Zhang, Pascal Bigey, et al.. Coupling Electrochemical Adsorption and Long‐range Electron Transfer: Label‐free DNA Mismatch Detection with Ultramicroelectrode (UME). Electroanalysis, 2019, 31 (11), pp.2232-2237. ⟨10.1002/elan.201900357⟩. ⟨hal-02189989⟩